Journal: Scientific Reports

Article Title: Genetic editing of primary human dorsal root ganglion neurons using CRISPR-Cas9

doi: 10.1038/s41598-025-91153-2

Figure Lengend Snippet: Lipofectamine 3000 reagent protocol. The hDRG cells were transfected according to these optimized specifications.

Article Snippet: BrainPhys ® media (Stemcell technologies, cat. no. 05790), 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15070063), 1% GlutaMAX ® (United States Biological, cat. no. 235242), 2% NeuroCultTM SM1 (Stemcell technologies, cat. no. 05711), 1% N-2 Supplement (Thermo Scientific, cat. no. 17502048), 2% HyCloneTM Fetal Bovine Serum (ThermoFisher Scientific SH3008803IR), 1: 1000 FrdU, 10 ng/mL Human β nerve growth factor - (Cell Signaling Technology, Cat# 5221SC).

Techniques: Transfection

Journal: Cell Stem Cell

Article Title: Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer’s patients

doi: 10.1016/j.stem.2021.04.004

Figure Lengend Snippet:

Article Snippet: BrainPhys culture media , StemCell Technologies , Cat#05790.

Techniques: Virus, Recombinant, Knock-Out, Fluorescence, Cell Culture, Imaging, SYBR Green Assay, Sample Prep, Protease Inhibitor, Lysis, Extraction, Enzyme-linked Immunosorbent Assay, Generated, Software, Functional Assay